directly spotted onto a membrane (nitrocellulose or nylon). The membrane is then probed with a labeled. A nitrocellulose membrane, approximately the size of the gel, presoaked in Western Transfer Buffer* for five minutes. (Note: The membrane should be handled.. nitrocellulose membrane, nylon membrane, other purous membrane, non-porous membrane, a plurality of polymeric pins, or a plurality of microtitre wells... Hercules, CA) were loaded on a standard 10% Arizona Custom SDS polyacrylamide gel, and fractionated proteins were electroblotted onto a nitrocellulose membrane.. For
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